Bioluminescent Whole-cell Biosensor for On-line Water Toxicity Detection
نویسندگان
چکیده
We present a novel microbial whole-cell sensing system for biodefense and environmental pollution monitoring. Real time water toxicity monitoring is achieved using genetically engineered E. coli bioluminescent cells which emit light when exposed to toxic materials. The illumination intensity and its time dependence are a function of the toxicants dose and type. The bacteria are immobilized in a disposable PDMS biochip that provides live cell maintenance and micro fluidics channels for sample injection. Several bacteria strains have been developed, generating a unique response signature for different toxins.
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